In vitro test


Global Leader of Human Skin & Bio Research
  • 17
    • Cytotoxicity Test

      The cytotoxicity of the cosmetics ingredients and natural substances is determined by MTT, XTT, WST-1 and Alamar blue assay

    • Duration

      6 weeks

  • 16
    • Collagen synthesis assay

      The effect of cosmetics ingredients and natural substances on collagen synthesis is evaluated in human dermal fibroblast with procollagen biosynthesis with Procollagen Type I C-peptide EIA kit in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics?intercellular collagen synthesis assay

    • Duration

      10 weeks

  • 15
    • Collagenase inhibition assay

      The effect of cosmetics ingredients and natural substances on collagenase inhibition is evaluated in cells induced collagenase by ELISA assay in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics-Intracellular collagenase activity inhibition test

    • Duration

      10 weeks

  • 14
    • Elastase inhibition assay

      The effect of cosmetics ingredients and natural substances on elastase inhibition is determined by enzyme assay using elastase from human dermal fibroblast cells in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics- elastase inhibition assay

    • Duration

      4 weeks

  • 13
    • Tyrosinase inhibition assay

      The effect of cosmetics ingredients and natural substances on tyrosinase activity is evaluated by in vitro enzyme assay using mushroom tyrosinase and L-tyrosine as a substrate in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics- In vitro tyrosinase inhibition test

    • Duration

      4 weeks

  • 12
    • DOPA oxidation inhibition assay

      The inhibitory effect of cosmetics ingredients and natural substances on DOPA oxidation is evaluated by in vitro enzyme assay using mushroom tyrosinase and L-DOPA as a substrate in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics- In vitro DOPA oxidation inhibition test

    • Duration

      4 weeks

  • 11
    • Melanin synthesis inhibition assay

      The effect of cosmetics ingredients and natural substances on melanin synthesis inhibition is determined in B16F10 cells stimulated with melanin elevating agents in accordance with the Ministry of Food and Drug Safety guideline for evaluation of the effectiveness of functional cosmetics-melanin synthesis inhibition test

    • Duration

      10 weeks

  • 10
    • Anti-oxidant assay (DPPH)

      The effect of cosmetics ingredients and natural substances on anti-oxidant activity is determined by the DPPH radical scavenging method

    • Duration

      4 weeks

  • 9
    • Oxygen Radical Absorbance Capacity assay (ORAC)

      Oxygen radical absorbance capacity (ORAC) is a method of measuring antioxidant capacities in cosmetics ingredients and natural substances in vitro. The effect of sample on anti-oxidant activity is determined by the ROO? (peroxyl radical) scavenging method

    • Duration

      6 weeks

  • 8
    • Total polyphenol content assay

      Total polyphenol content is determined using a spectrophotometric assay based on the reduction of Folin-Ciocalteau reagent by phenolic compounds, with a concomitant formation of a blue complex

    • Duration

      4 weeks

  • 7
    • Nitrogen oxide (NO) assay

      The inhibitory effect of cosmetics ingredients and natural substances on NO production is evaluated in LPS-induced Raw264.7 cells

    • Duration

      8 weeks

  • 6
    • Western blot

      The effect of cosmetics ingredients and natural substances on the specific protein related to inflammation, skin aging, skin barrier and hydration is evaluated by western blot, an analytical technique used to detect the specific protein from a cell extract

       

       Efficacy  Factor (cytokine)
       Anti-inflammation  IL-1α, IL-1β, IL-6, IL-8, TNF-α, COX-2
       Anti-aging  Collagen type I
       MMP-1 (collagenase)
       MMP-2 (gelatinase)
       MMP-3 (Stromelysin)
       MMP-9 (gelatinase)
       TIMP-1 (inhibitor of the MMPs)
       Skin barrier & hydration 
       Involucrin, Filaggrin, AQP-3, HAS-2, Loricrin, Keratin  

    • Duration

      12 weeks

  • 5
    • RT-PCR & RT-qPCR

      The effect of cosmetics ingredients and natural substances on mRNA level of genes related to inflammation, skin aging, skin barrier and hydration is evaluated using RT-PCR & RT-qPCR

    • Duration

      12 weeks

  • 4
    • ELISA assay

      The effect of cosmetics ingredients and natural substances on the specific inflammatory cytokines related to inflammation is evaluated by ELISA assay

    • Duration

      10 weeks

  • 3
    • Alternative test using artificial skin (non-GLP)

      Skin initiaion of the test material is evaluated by skin irritation test using reconstructed human epidermis in accordance with the OECD guideline (TG 439) in Ministry of Food and Drug Safety guideline

    • Duration

      12 weeks

  • 2
    • in vitro Skin absorption method

      The effect of cosmetics ingredients and natural substances on Skin absorption is evaluated using Franz diffusion cell in accordance with the Ministry of Food and Drug Safety guideline for in vitro Skin absorption method

    • Duration

      8 weeks

  • 1
    • 3T3 NRU Phototoxicity Test

      The effect of cosmetics ingredients and natural substances on phototoxicity is evaluated using 3T3 NRU assay in accordance with the Ministry of Food and Drug Safety guideline for in vitro 3T3 NRU phototoxicity Test

    • Duration

      8 weeks

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